FluoroGold

FluoroGold Doses

i.p. 1 mg/1 ml (0.1% solution = 10 mg/10 ml)

Brain: 0.25 µl (1% solution = 100mg/10 ml)

Gut wall: 10 µl (4% solution = 400mg/10ml)

50 mg/tube/1.25 ml = 4% solution = 1250 µl of 4% solution

Aliquot into 50 tubes of 25 µl each of 4% Fluorogold in saline.

Dilute 25µl aliqout by (FACTOR) by adding (SALINE) to final volume of (VOLUME) for (CONCENTRATION)

Factor	Saline	Volume	Concentration
40	975 µl	1000 µl	0.1%
4	75 µl	100 µl	1%
0	0	25 µl	4%

Vagotomy Confirmation

Wait 3-5 days after i.p. injection, then perfuse and process for FG immuno using Chemicon antibody at 1:5000. Use standard immuno protocol with rabbit secondary antibody, ABC kit, and 5-min DAB reaction.