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==DNA Methylation sites in c-Fos promoter==
Approximate MW:
38,337 daltons (unmethylated)
38,252 daltons (methylated)
each methylation site causes 15 dalton decrease in MW

From Shimizu et al., Cancer Sci 2007; 98: 1318–1322
==Rat c-fos Primers==
'''Bisulfite sequencing (Genomic DNA)'''
GenBank AF126534
F: 5′-TATTTATAGGTGAAAGTTATAGATTG-3′
R: 5′-CACTAATAAAAACTACAAAACAAAACT-3′
Annealing temperature 54
Product size 292 bases
''4-23-08 -- could NOT confirm with blast -- need to check earlier references''
'''RT-PCR (from RNA)'''
GenBank X06769
F: 5′-TTGCGCAGATCTGTCCGTCT-3′
R: 5′-GTTGATCTGTCTCCGCTTGG-3′
Annealing temperature 65
''4-23-08 -- confirmed with blast''
Approximate MW:
38,337 daltons (unmethylated)
38,252 daltons (methylated)
each methylation site causes 15 dalton decrease in MW

From Shimizu et al., Cancer Sci 2007; 98: 1318–1322
==Rat c-fos Primers==
'''Bisulfite sequencing (Genomic DNA)'''
GenBank AF126534
F: 5′-TATTTATAGGTGAAAGTTATAGATTG-3′
R: 5′-CACTAATAAAAACTACAAAACAAAACT-3′
Annealing temperature 54
Product size 292 bases
''4-23-08 -- could NOT confirm with blast -- need to check earlier references''
'''RT-PCR (from RNA)'''
GenBank X06769
F: 5′-TTGCGCAGATCTGTCCGTCT-3′
R: 5′-GTTGATCTGTCTCCGCTTGG-3′
Annealing temperature 65
''4-23-08 -- confirmed with blast''