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Changes
corrected enzyme tube number
Add:
Add:
*1 µl each dCTP, dGTP, dTTP (tubes 3,4,5)
*1 µl each dCTP, dGTP, dTTP (tubes 3,4,5)
*4 µl of 5x Klenows enzyme/hexanucleotide mixture (tube 6)
*4 µl of 5x Klenows enzyme/hexanucleotide mixture (tube 1)
*5 µl 35S-dATP
*5 µl 35S-dATP