Changes

#10% TCA washing----use a top of  the pipette tip boxes for P1000 filtered tips. Three washes for 5min, 5min, 30min each with gentle agitation by hands. When you change washes you can just pour out, the filters will stick on the plastic box. Place the filters in the scintillation vials and add the cocktail. TCA is dangerous, be careful not to burn your skin while you handle it!!!

#10% TCA washing----use a top of  the pipette tip boxes for P1000 filtered tips. Three washes for 5min, 5min, 30min each with gentle agitation by hands. When you change washes you can just pour out, the filters will stick on the plastic box. Place the filters in the scintillation vials and add the cocktail. TCA is dangerous, be careful not to burn your skin while you handle it!!!

#you can keep your RT tube in the refigerator if you’re gonna use it at the same day, otherwise keep it in the freezer.

#you can keep your RT tube in the refigerator if you’re gonna use it at the same day, otherwise keep it in the freezer.