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Design of a viral vector for knocking down Yellow Fluorescent Protein expression (and whatever it is attached to).
Design of a viral vector for knocking down Yellow Fluorescent Protein expression (and whatever it is attached to).
Virus will be constructed and produced by the [http://pgtc.pediatrics.med.ufl.edu/core-facilities/vector-core-lab UF Vector Core Lab].
To rescue limited brain regions in the FKO mice, we will construct a rAAV virus which will express a short-hairpin RNA (shRNA) that causes degradation of YFP, using previously published shYFP sequences PMID 16676321 PMID 18708075. PMID 15582578 ( Targeted degradation of the 771-nt YFP portion of a YFP-tagged transgene should be sufficient to block expression of the complete transgene PMID 16676321.
To rescue limited brain regions in the FKO mice, we will construct a rAAV virus which will express a short-hairpin RNA (shRNA) that causes degradation of YFP, using previously published shYFP sequences PMID 16676321 PMID 18708075. PMID 15582578 ( Targeted degradation of the 771-nt YFP portion of a YFP-tagged transgene should be sufficient to block expression of the complete transgene PMID 16676321.