Changes

The construct will also contain DsRed or mCherry as the reporter gene to allow co-localization with the constitutive YFP reporter in 14-3-3 FKO mice.  A rAAV-DsRed vector without shYFP will be constructed as a control virus.

The construct will also contain DsRed or mCherry as the reporter gene to allow co-localization with the constitutive YFP reporter in 14-3-3 FKO mice.  A rAAV-DsRed vector without shYFP will be constructed as a control virus.

Oligonucleotides encoding the sense and antisense strands of shRNA against GFP/YFP PMID 15582578:

Oligonucleotides encoding the sense and antisense strands of shRNA against GFP/YFP PMID 15582578:

siGFP sense: 5’-gat ccc gca agc tga ccc tga agt tca ttt caa gag aat gaa ctt cag ggt cag ctt gct ttt ttg gaa a-3’

siGFP sense: 5’-gat ccc gca agc tga ccc tga agt tca ttt caa gag aat gaa ctt cag ggt cag ctt gct ttt ttg gaa a-3’

siGFP anti-sense: 5’-agc ttt tcc aaa aaa gca agc tga ccc tga agt tca ttc tct tga aat gaa ctt cag ggt cag ctt gcg a-3’

siGFP anti-sense: 5’-agc ttt tcc aaa aaa gca agc tga ccc tga agt tca ttc tct tga aat gaa ctt cag ggt cag ctt gcg a-3’

used to construct SiRNA vector pAAV9(5)-hSyn-DsRed2N1-EGFP-siRNA gb|AY640634.1 [http://www.ncbi.nlm.nih.gov/nucleotide/56119203?report=genbank GenBank AY640634.1]