1,093
edits
Changes
updated references, R18 sequence
==Dr. Yi Zhou's Thy1-R18 Transgenic Mice==
==14-3-3==
http://ghr.nlm.nih.gov/gene/YWHAE
Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)
Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA
NCBI Reference Sequence: NM_009536.4
==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-R18) Transgenic Mice==
See SFN abstracts (2010) 53.1/M17 and (2011) 790.09/FF16
See SFN abstracts (2010) 53.1/M17 and (2011) 790.09/FF16
R18 peptide paper: PMID 10493820
==R18 peptide ==
PMID 10493820 PMID 11577088
Thy-1 Promoter paper: PMID 11086982
R18 (aka difopein) is a synthetic unphosphorylated peptide (MW 2309.6) which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3.
Sequence:
Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala- Asn-Met-Cys-Leu-Pro-OH
PHCVPRDLSWLDLEANMCLP
Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/]
==Thy-1 Promoter==
PMID 11086982
==YFP==
YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. R18 is 21 amino acids long (63 bp), so total YFP-R18 fusion protein should be ~ 260 amino acids (780 bp).
YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. R18 is 21 amino acids long (63 bp), so total YFP-R18 fusion protein should be ~ 260 amino acids (780 bp).
== 14-3-3 FKO papers==
Vogt, Peggy, [http://diginole.lib.fsu.edu/uhm/42/ "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.
Vogt, Peggy, [http://diginole.lib.fsu.edu/uhm/42/ "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.
C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400
C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400
Qiao H, Foote M, Graham K, Wu Y, Zhou Y. 14-3-3 proteins are required for hippocampal long-term potentiation and associative learning and memory. J Neurosci. 34(2014):4801-8. PMID 24695700