− | YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. R18 is 21 amino acids long (63 bp), so total YFP-R18 fusion protein should be ~ 260 amino acids (780 bp). | + | YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp). |