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DME Sodium Butyrate and Flavor Conditioning 2 (view source)
Revision as of 10:51, 8 September 2020
, 10:51, 8 September 2020added CTA experiment agenda
Durba Mukherjee
Durba Mukherjee
==Agenda==
==CFP Conditioning==
===Agenda===
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==CFP Retest==
===Agenda===
==CTA Positive Control==
===Agenda===
'''Day 0:''' (August 30, 2020) Water removed, ad lib food access.
'''Day 1-9:''' (August 31-Sept 8, 2020) the rats were placed on a water deprivation schedule under which they received daily water access in one 30-min drinking session. Rats had ad lib food access throughout.
'''Day 10: Conditioning day''' (Sept 9, 2020): rats were divided into 4 different groups
(NaCl/NaCl, NaCl/NaB, LiCl/NaCl and LiCl/NaB)
0-10 min: 10-min access to a bottle containing 75 mM NaCl solution (half-isotonic, 4.5g NaCl/liter).
30 min: (20 minutes after the end of NaCl) intake:
* LiCl/NaCl and LiCl/NaB groups were injected with LiCl (0.15 M, 3 ml/kg, i.p.)
* NaCl/NaCl and NaCl/NaB groups were injected with saline (0.15 M NaCl, 3 ml/kg, i.p.).
40 min: (10 minutes after LiCl or saline injections)
* LiCl/NaB and NaCl/NaB groups were injected with NaB (0.3 M, 12 ml/kg, i.p.)
* LiCl/NaCl and NaCl/NaCl groups were injected with iso-osmotic saline (0.3 M NaCl, 12 ml/kg, i.p.).
3 h: (3 hours after conditioning) rats received ad libitum access to water overnight.
'''Day 11''' (Sept 10, 2020): CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 75 mM NaCl and water bottles, alternating position for 6 days.
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[[Category:Flavor Preference]]
[[Category:Flavor Preference]]