− | Following Jongen-Relo, A. L., & Feldon, J. (2002) PMID 12126979 and in the gustatory cortex, Geddes et al. (2008) PMID 18823161, we will determine loss of neurons using a mouse monoclonal [http://www.emdmillipore.com/US/en/product/,MM_NF-MAB377 anti-NeuN, clone A60], from Millipore, catalog MAB377, price $259.00. Jongen-Relo used a primary concentration of 1:5000 with a 48-h incubation at 4 C. | + | Following Jongen-Relo, A. L., & Feldon, J. (2002) PMID 12126979 and in the gustatory cortex, Geddes et al. (2008) PMID 18823161, we will determine loss of neurons using a mouse monoclonal [https://www.emdmillipore.com/US/en/product/Anti-NeuN-Antibody-clone-A60,MM_NF-MAB377 anti-NeuN, clone A60], from Millipore, catalog MAB377, price $259.00. Jongen-Relo used a primary concentration of 1:5000 with a 48-h incubation at 4 C. |