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== FluoroGold Doses ==
i.p. 1 mg/1 ml (0.1% solution = 10 mg/10 ml)
Brain: 0.25 µl (1% solution = 100mg/10 ml)
Gut wall: 10 µl (4% solution = 400mg/10ml)
50 mg/tube/1.25 ml = 4% solution = 1250 µl of 4% solution
Aliquot into 50 tubes of 25 µl each of 4% Fluorogold in saline.
Dilute 25µl aliqout by (FACTOR) by adding (SALINE) to final volume of (VOLUME) for (CONCENTRATION)
<tab class="wikitable" sep="tab" border = "1" cellspacing="3" cellpadding = "3" head = top>
Factor Saline Volume Concentration
40 975 µl 1000 µl 0.1%
4 75 µl 100 µl 1%
0 0 25 µl 4%
</tab>
== Vagotomy Confirmation==
Wait 3-5 days after i.p. injection, then perfuse and process for FG immuno using Chemicon antibody at 1:5000. Use standard immuno protocol with rabbit secondary antibody, ABC kit, and 5-min DAB reaction.
[[Category:Protocols]]