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JCE Inhibitory DREADD Functional Validation (view source)
Revision as of 15:32, 6 August 2023
, 15:32, 6 August 2023no edit summary
To test CNO as a uncondition stimulus for CTA in wild-type mice.
CNO ordering information / lot numbers
CNO preparation (e.g. vehicle, how its dissolved)
{{Infobox experiment|align=right
| Name = Clozapine-N-Oxide Taste Aversion
| project = DREADDs and Magentic Field
| code = JCD
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Cote, Houpt
| species = mice
| date = 2023-6-19
| type = CTA
| drugs = NaCl, CNO
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=JCD
}}
==Data==
'''[https://houptlab.org/bartab/expt/?id=JCD Raw Data Link]'''
==People==
Jason Cote
==Agenda==
Mice JAX C57BL/6J Strain 000664 (n=8 male DOB +/- 3: 20Dec2022, n=8 female DOB +/- 3: 20Dec2022)
Arrived 2023-2-6.
'''Day 0: Water removed''' (2023-6-19)
'''Days 1 - 11: Water Restriction''' (2023-6-20 - 2023-6-30)
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.
Mice given water bottle and empty bottle, alternating position.
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.
'''Day 12: Conditioning Day''' (2023-3-16)
Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg
Groups:
* JCC01-04: Female, vehicle
* JCC05-08: Female, CNO
* JCC09-12: Male, vehicle
* JCC13-16: Male, CNO
After injection, ad lib water returned overnight.
'''Day 13 - 24: Preference Tests''' (2023-3-17 - 2023-3-24 )
CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Magnet Experiments]]