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JCE Inhibitory DREADD Functional Validation (view source)
Revision as of 18:16, 9 August 2023
, 18:16, 9 August 2023→Agenda
'''Day 0: Water removed''' (2023-6-19)
'''Day 0: Water removed''' (2023-8-26)
'''Days 1 - 11: Water Restriction''' (2023-6-20 - 2023-6-30)
'''Days 1 - 11: Water Restriction''' (2023-8-27 - 2023-9-06)
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.
'''Day 12: Conditioning Day''' (2023-3-16)
'''Day 12: Conditioning Day''' (2023-3-16)
Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) after 10 min