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Examine levels of phosphorylated pyruvate dehydrogenase by immunofluorescence in after intraoral conditioned taste aversion.
Examine levels of [[Phospho-Pyruvate Dehydrogenase (pPDH)]] by immunofluorescence in after intraoral conditioned taste aversion.
Based on Yang et al. 2024 PMID 38266644 and report by Roitman lab at SSIB 2024.
Based on Yang et al. 2024 PMID 38266644 and report by Roitman lab at SSIB 2024.
Employed SD-GFP-TH rats (Iacovitti et al 2014 PMID 25462571) from Taconic that express GFP under TH promoter with especially good expression in VTA.
Employed SD-GFP-TH rats (Iacovitti et al 2014 PMID 25462571) from Taconic that express GFP under TH promoter with especially good expression in VTA.
For immunohistochemistry:
* for pPDH: [https://www.cellsignal.com/products/primary-antibodies/phospho-pyruvate-dehydrogenase-a1-ser293-e4v9l-rabbit-mab/37115 Phospho-Pyruvate Dehydrogenase α1 (Ser293) (E4V9L) Rabbit mAb #37115] at 1:500 for immunofluoresence; BLUE secondary [https://www.jacksonimmuno.com/catalog/products/711-475-152 Jackson DyLight™ 405 AffiniPure™ Donkey Anti-Rabbit IgG (H+L) #711-475-152] [WHAT IS CONCENTRATION?]
* for c-Fos: GET Antibody specification and secondary from Jason's work
{{Infobox experiment|align=right
{{Infobox experiment|align=right