1,145
edits
Changes
fixed TSS calculation
Linker DNA between nucleosomes 10-80 bp
Linker DNA between nucleosomes 10-80 bp
ideal MNase mononucleosomal digest -> 3 x 10<sup>7</sup> fragments per cell (3 x 10<sup>13</sup> for 1 million cells); if 4 x 10<sup>5</sup> transcription start sites (TSS), then ~ 0.1% of mononucleosomal fragments will be of interest.
ideal MNase mononucleosomal digest -> 3 x 10<sup>7</sup> fragments per cell (3 x 10<sup>13</sup> for 1 million cells); if 4 x 10<sup>5</sup> transcription start sites (TSS) of 2000 bp length, so ~10 nucleosomes/TSS, then ~ 4 x10<sup>6</sup> (~10%) of mononucleosomal fragments will be of interest.
Acetylation sites: "On average, 5 to 9 lysine residues of H2B, H3, and H4 were acetylated while a higher number (10 to 11 sites) of modifications was observed for H2A [but may be more]" PMID 37011222 ; so total of 50 - 76 acetylation sites per nucleosome.
Acetylation sites: "On average, 5 to 9 lysine residues of H2B, H3, and H4 were acetylated while a higher number (10 to 11 sites) of modifications was observed for H2A [but may be more]" PMID 37011222 ; so total of 50 - 76 acetylation sites per nucleosome.