1,150
edits
Changes
added adrenal link and number of cells
* transcardial perfusion 100ml/2min Saline nitride heparine for each rat
* transcardial perfusion 100ml/2min Saline nitride heparine for each rat
* transcardial perfusion with 400ml/4min 1% phospahte buffered paraformaldehyde
* transcardial perfusion with 400ml/4min 1% phospahte buffered paraformaldehyde
* Dissect rat adrenal gland and put in PBS on ice and tranfer to the lab
* Dissect [[Arenal_Gland rat adrenal gland]] and put in PBS on ice and tranfer to the lab
* Separate medulla in PBS (the tissues were in PBS around 60 min)
* Separate medulla in PBS (the tissues were in PBS around 60 min)
* 4 medullas in Dounce homogenizer in 1ml PBS,
* 4 medullas (~4 x 10<sup>6</sup> cells total) in Dounce homogenizer in 1ml PBS,
* Quenching by adding with 1/20 of stock Glycine (2.5M), final concentration is 125mM
* Quenching by adding with 1/20 of stock Glycine (2.5M), final concentration is 125mM
* homogenize the tissues and let the tube sit for 5 min at RT
* homogenize the tissues and let the tube sit for 5 min at RT