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Test if lithium orotate (LiOr) induces a conditioned taste aversion comparable to LiCl.
Single pairing of saccharin intake (15 min) after 15-min delay with isomolar doses of LiCl and LiOr to see if LiOr is more potent.
{{Infobox experiment|align=right
| Name = Lithium Orotate and CTA pt2
| project = Taste Aversion
| code = SNB
| protocol = 20220000006
| funding = Chair's Fund
| investigators = Nabi, Houpt
| species = rat
| date = 2026-3-13
| type = CTA
| drugs = lithium orotate, lithium chloride
| data = https://houptlab.org/bartab/expt/?id=SNB
}}
== Data ==
[https://houptlab.org/bartab/expt/?id=SNB Raw Data Link]
== People ==
Soheila Nabi
==CTA Conditioning==
===Agenda===
Rats arrive (2026-2-10), n= 16 adult male Sprague Dawley rats (Charles River CD, d.o.b 2025-12-15), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (2026-3-12)
make labels for bottles.
<br>
'''Days 1 - 6: Water Restriction''' (2026-3-13 thru 2026-3-18)
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 900 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
<br>
'''Day 12: Conditioning Day''' (2026-3-19)
0 min: 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: rats injected with:
* 75 mM NaCl, 6 ml/kg ip. (SNB01,04,07,10,13)
* 75 mM LiCl, 6 ml/kg ip. (SNB02,05,08,11,14)
* 75 mM LiOr, 6 ml/kg ip. (SNB03,06,09,12,15,16)
all injections made isotonic with TrisAmino
ad lib water returned after final injection overnight.
'''Days 13 - 22: Preference Tests''' (2026-3-20 through 2026-3-31)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
Single pairing of saccharin intake (15 min) after 15-min delay with isomolar doses of LiCl and LiOr to see if LiOr is more potent.
{{Infobox experiment|align=right
| Name = Lithium Orotate and CTA pt2
| project = Taste Aversion
| code = SNB
| protocol = 20220000006
| funding = Chair's Fund
| investigators = Nabi, Houpt
| species = rat
| date = 2026-3-13
| type = CTA
| drugs = lithium orotate, lithium chloride
| data = https://houptlab.org/bartab/expt/?id=SNB
}}
== Data ==
[https://houptlab.org/bartab/expt/?id=SNB Raw Data Link]
== People ==
Soheila Nabi
==CTA Conditioning==
===Agenda===
Rats arrive (2026-2-10), n= 16 adult male Sprague Dawley rats (Charles River CD, d.o.b 2025-12-15), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (2026-3-12)
make labels for bottles.
<br>
'''Days 1 - 6: Water Restriction''' (2026-3-13 thru 2026-3-18)
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 900 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
<br>
'''Day 12: Conditioning Day''' (2026-3-19)
0 min: 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: rats injected with:
* 75 mM NaCl, 6 ml/kg ip. (SNB01,04,07,10,13)
* 75 mM LiCl, 6 ml/kg ip. (SNB02,05,08,11,14)
* 75 mM LiOr, 6 ml/kg ip. (SNB03,06,09,12,15,16)
all injections made isotonic with TrisAmino
ad lib water returned after final injection overnight.
'''Days 13 - 22: Preference Tests''' (2026-3-20 through 2026-3-31)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]