Changes

SNE Lithium Orotate Inhibitors 2 (view source)

84 bytes added , 14 July

updated DMSO, weighing, some dates

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* measure 400mg naringin + 120mg 6-azuracil;

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* dissolve in ~~4ml~~ DMSO with heavy vortexing

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* dissolve in '''2ml''' DMSO with heavy vortexing

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* slowly add 36 ml sterile injectable 0.9% saline, with frequent vortexing (-> clear light yellow solution)

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* slowly add '''38''' ml sterile injectable 0.9% saline, with frequent vortexing (-> clear light yellow solution)

* filter

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Use 10% DMSO in injectable 0.9% saline as vehicle control.

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Use '''5%''' DMSO in injectable 0.9% saline as vehicle control.

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''(Note: used only 5% DMSO, while SND used 10% DMSO)''

{{Infobox experiment|align=right

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<br>

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'''Days 1 - 6: Water Restriction''' (2026-7-8 thru 2026-6-13)

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'''Days 1 - 6: Water Restriction''' (2026-7-8 thru 2026-7-13)

Weigh rats daily. ad lib food.

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'''Day 7: Conditioning Day''' (2026-7-14)

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-30 min (~930): rats injected with naringin (100mg/kg) + 6-azauracil (30mg/kg) or saline vehicle, 10 ml/kg ip

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-30 min (~930): rats weighed and injected with naringin (100mg/kg) + 6-azauracil (30mg/kg) or saline vehicle, 10 ml/kg ip

0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)

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