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SNF Lithium Orotate Inhibitors 3 (view source)
Revision as of 13:01, 12 September 2026
, 12 Septembercreated page
Continuation of [[SNA Lithium Orotate CTA]] and [[SNB Lithium Orotate CTA 2]] and [[SNC Non-Contingent Lithium Orotate]] [[SND Lithium Orotate Inhibitors]] [[SNE Lithium Orotate Inhibitors 2]]
Test if OAT inhibitor naringin and UMPS inhibitor 6-azauracil decrease effectiveness of lithium orotate (LiOr) in inducing a conditioned taste aversion.
Inject narinign and 6-azauracil 30 min before single pairing of saccharin intake (15 min) after 15-min delay with LiOr.
From Pacholko 2023 PMID 37356352:
naringin MW 580.53 [https://www.sigmaaldrich.com/US/en/product/sigma/71162 Sigma 71162-35g] $50.78: organic anion transporting polypeptide 1A2 (OATP1A2) inhibitor
6-azauracil MW 113.07 [https://www.sigmaaldrich.com/US/en/product/sigma/a1757 Sigma A1757-5g] $99.90: UMPS inhibitor
For injection, combine 100 mg/kg naringin (17mM) + 30 mg/kg 6-azauracil (27mM) dissolved in saline/10% DMSO; in 10 ml/kg i.p. injection.
''Note: Mix with saline on day of injection, otherwise will precipitate overnight.''
* measure 400mg naringin + 120mg 6-azuracil;
* dissolve in '''2ml''' DMSO with heavy vortexing
* slowly add '''38''' ml sterile injectable 0.9% saline, with frequent vortexing (-> clear light yellow solution)
* filter
Use '''5%''' DMSO in injectable 0.9% saline as vehicle control.
''(Note: used only 5% DMSO, while SND used 10% DMSO)''
{{Infobox experiment|align=right
| Name = Lithium Orotate Inhibitors
| project = Taste Aversion
| code = SNF
| protocol = 20220000006
| funding = Chair's Fund
| investigators = Nabi, Houpt
| species = rat
| date = 2026-9-12
| type = CTA
| drugs = lithium orotate, lithium chloride
| data = https://houptlab.org/bartab/expt/?id=SNf
}}
== Data ==
[https://houptlab.org/bartab/expt/?id=SNf Raw Data Link]
== People ==
Soheila Nabi
==CTA Conditioning==
===Agenda===
Rats arrive (2026-XXX), n=16 adult male Sprague Dawley rats (Charles River CD, d.o.b 2026-XXXX), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (2026-9-13)
make labels for bottles.
<br>
'''Days 1 - 6: Water Restriction''' (2026-9-14 thru 2026-9-19)
Weigh rats daily. ad lib food.
30 min, 30 min, 30 min, 30 min, 30 min, 15 min water at 1000 daily.
1 water bottle, 1 empty bottle to accustom to 2-bottle choice
<br>
'''Day 7: Conditioning Day''' (2026-9-20)
-30 min (~930): rats weighed and injected with naringin (100mg/kg) + 6-azauracil (30mg/kg) or saline vehicle, 10 ml/kg ip
0 min (~1000): all rats 15 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min (~1030): 6 rats injected with 75 mM LiOr, 6 ml/kg ip, or Tris-NaCl vehicle.
Thus, four groups:
* Veh-Veh (SNF01,05,09,13)
* NA-Veh (SNF02,06,10,14)
* Veh-LiOr (SNF03,07,11,15)
* NA-LiOr (SNF04,08,12,16)
Ad lib water returned after final injection overnight.
'''Days 8 - 22: Preference Tests''' (2026-9-21 through 2026-10-06)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 14 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]