Serotonin Genes

I am interested in 4 of the genes related to serotonin function in depression and food intake:

Pet-1

Serotonin Transporter

Tryptophan Hydroxylase 2

5HT2C Receptor

PCR primers

Tryptophan Hydroxylase 2 cDNA for in situ hybridization

product size: bp, range & ascension number?

TPH2for GAAGTTGGCGGGCTGGTGAGA 21 bases TPH2rev TCGGCAAGCATGAGTGGGGTAGA 23 bases

Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992

Tryptophan Hydroxylase 2 polymorphism linked to aggression

Positive control, product size 523 bp ctrlTPH2for: 5’ -tttgacccaaagacgacctgcttgca –3’ ctrlTPH2rev: 5’-tgcatgcttactagccaaccatgagaca-3’

1473C allele: 307bp TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’

1473G allele : 307 bp TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’

Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473