C-Fos Methylation

DNA Methylation sites in c-Fos promoter

Approximate MW:

38,337 daltons (unmethylated)

38,252 daltons (methylated)

each methylation site causes 15 dalton decrease in MW 

From Shimizu et al., Cancer Sci 2007; 98: 1318–1322

Rat c-fos Primers

Bisulfite sequencing (Genomic DNA)

GenBank AF126534

F: 5′-TATTTATAGGTGAAAGTTATAGATTG-3′

R: 5′-CACTAATAAAAACTACAAAACAAAACT-3′

Annealing temperature 54

Product size 292 bases

4-23-08 -- could NOT confirm with blast -- need to check earlier references

RT-PCR (from RNA)

GenBank X06769

F: 5′-TTGCGCAGATCTGTCCGTCT-3′

R: 5′-GTTGATCTGTCTCCGCTTGG-3′

Annealing temperature 65

4-23-08 -- confirmed with blast