RT-PCR Conditions for a bunch of genes implicated in Bipolar Disorder

DAAO

Source: Konno 1998 PMID 12734589

Sense primer (F: 5’-GGTTAACTGAGAGGGGAGTGAA-3’)

Antisense primer (R: 5’- CCATAGTTGTGGATGACCTCTG-3’)

designed from the sequence conserved in cDNAs encoding human, mouse, rabbit, and pig D-amino-acid oxidases.

initial denaturation at 94°C for 1 min, 30 cycles of PCR

denaturation: 94°C, 30 sec

annealing: 55°C, 30 sec

extension: 72°C, 45 sec)

final extension was done at 72°C for 5 min

Serine Racemase