DME Sodium Butyrate and Flavor Conditioning 2

Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances preference learning

Replication of [DMC Sodium Butyrate and Flavor Conditioning] with following modifications:

1. To prevent formation of side preferencs, rats were trained with 2 choice water and 0.05% saccharin prior to conditioning

2. To prevent ceiling effect of preferences being too strong, CS+ used only 4% glucose

3. n=9 male, n=9 female, counterbalanced

Data

Durba Mukherjee

Rats arrive. (July 7, 2020)

n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169.

Days 0: Food and water removed. (July 10, 2020)

Days 1: Food and water restricted. (July 11 - July 17, 2020)

Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.

30 min water at 1100am daily, pellet ration at 1130am

Day 9: Conditioning Day 1 (July 18, 2020)

0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am

30 min: remove CS+, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, 1200am-1213am

Give daily pellet ration at 1215am

Day 10: Conditioning Day 2 (July 19, 2020)

0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am

CS- bottles not weighed, so estimated volumetrically from fill lines.

30 min: remove CS-, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl,

return food and water ad lib

Days 11-17: 2 bottle preference tests (July 20 - 28, 2020)

Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.