DMJ Valproate and Saccharin CTA
Test if pre-conditioning injection of sodium valproate enhances conditioned taste aversion induced by low dose of LiCl in females vs. male rats
Data
People
Durba Mukherjee
CTA Conditioning
Agenda
Rats arrive (March 16, 2021) n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed (March 22, 2021)
Days 1 - 10: Water Restriction (March 23 - April 2, 2021)
Weigh rats daily. Water for 30 min, ad lib food.
30 min water at 1430 daily.
Day 11: Conditioning Day (April 3 2021)
-15 min: half of rats injected with 0.15M Na Valproate 8m/kg = 200ml/kg; other half 0.15M NaCl 8ml/kg 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
ad lib water returned after final injection overnight.
- rats 1-8 : female, Rats 9-18: male
- rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
- All rats: saccharin
- odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl
Days 12 - ??: Preference Tests (April 4 - ?? March , 2021)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.