DMT Ovariectomy and Butyrate-Enhanced CTA 3
Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of DMP Ovariectomy and Butyrate-Enhanced CTA.
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
Agenda
Rats arrive Female Sprague Dawley rats n=8 (DMT1-5: dob 2021-9-21; rcvd 2021-12-7. DMT6-8: dob 2021-10-25;rcvd 2022-1-11
Surgery
all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
- DMT 1-4 : Surgery on 2022-1-18
- DMT 5-8 : Surgery on 2022-1-19
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
Day 0: Water removed 2022-1-27
Days 1-8: Water Restriction 2022-1-28 thru 2022-2-4
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 9: Conditioning Day 2022-2-8
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
- at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
Groups:
- OVX + E + LiCl + NaCl: DMT 2,3,4,6
- OVX + E + LiCl + Butyrate: DMT 1,5,7,8
Days 10 - 19: Preference Tests: 2022-2-7 to 2022-2-16
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.