DMV Valproate 100mg/kg Controls
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Fill in some saline controls for Valproate enhancement of saccharin-LiCl CTA.
| DMV Valproate Controls | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMV |
| Start Date | 2022-12-1 |
| Investigators | Mukherjee, Bass, Houpt |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | valproate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMV |
Data
People
Marena Bass Durba Mukherjee (in absentia)
Agenda
Rats arrive (2202-11-22)
Sprague Dawley rats (dob males: 2022-???; females: 2022-???? )
Day 0: Water removed 2022-12-1
Days 1-8: Water Restriction 2022-12-2 thru 2022-12-9
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 9: Conditioning Day 2022-12-10
- -15 min: 8 ml/kg of 0.15M LiCl
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg ip of 0.15M LiCl or NaCl
Groups:
- DMV01-04 NaCl-Li-Male
- DMV05-08 NaCl-NaCl-Male
- DMV09-12 NaCl-Li-Female
- DMV13-16 NaCl-NaCl-Female
ad lib water returned after final injection overnight.
Days 10-21: Preference Tests: 2022-12-11 to 2022-12-23
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.