JCE Inhibitory DREADD Functional Validation
To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion.
CNO ordering information / lot numbers
CNO preparation (e.g. vehicle, how its dissolved)
| Clozapine-N-Oxide Taste Aversion | |
|---|---|
| DREADDs and Magentic Field | |
| Expt Code | JCE |
| Start Date | 2023-6-19 |
| Investigators | Cote, Houpt |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | mice |
| Drugs | NaCl, CNO |
| Data Link | https://houptlab.org/bartab/expt/?id=JCE |
Data
People
Jason Cote
Agenda
Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p), 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p), Sesame oil vehicle (10ml/kg i.p)
Pre Treatment 2 weeks before starting water restriction, 4 groups:
1. Isotonic LiCl (0.15M, 20ml/kg, i.p) and 4-OHT (50 mg/kg ip), N=6
2. Isotonic LiCl(0.15M, 20ml/kg, i.p) and sesame oil (10 ml/kg i.p), N=6
3. Isotonic saline (20ml/kg, i.p) and 4-OHT (50 mg/kg i.p), N=6
4. Isotonic saline (20ml/kg, i.p) and sesame oil (10ml/kg i.p), N=4
Day 0: Water removed (2023-6-19)
Days 1 - 11: Water Restriction (2023-6-20 - 2023-6-30)
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.
Mice given water bottle and empty bottle, alternating position.
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min. Day 12: Conditioning Day (2023-3-16)
Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg
Groups:
- JCC01-04: Female, vehicle
- JCC05-08: Female, CNO
- JCC09-12: Male, vehicle
- JCC13-16: Male, CNO
After injection, ad lib water returned overnight.
Day 13 - 24: Preference Tests (2023-3-17 - 2023-3-24 )
CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.