JCE Inhibitory DREADD Functional Validation

To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration: reducing/preventing taste aversion.

CNO ordering information / lot numbers

CNO preparation (e.g. vehicle, how its dissolved)

Clozapine-N-Oxide Taste Aversion
DREADDs and Magentic Field
Expt Code	JCE
Start Date	2023-6-19
Investigators	Cote, Houpt
Funding	Chair's Fund
Protocol	202200000006
Expt Type	CTA
Species	mice
Drugs	NaCl, CNO
Data Link	https://houptlab.org/bartab/expt/?id=JCE

Data

Raw Data Link

People

Jason Cote

Agenda

Mice In House Colony FosCreER X hM4Di (n=10 male DOB +/-, n=12 female DOB +/-)

Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p), 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p), Sesame oil vehicle (10ml/kg i.p)

Pre Treatment 2 weeks before starting water restriction, all injections are given concomitantly 4 groups:

1. Isotonic LiCl and 4-OHT, N=6

2. Isotonic LiCl and sesame oil, N=6

3. Isotonic saline and 4-OHT, N=6

4. Isotonic saline and sesame oil, N=4

Day 0: Water removed (2023-6-19)

Days 1 - 11: Water Restriction (2023-6-20 - 2023-6-30)

Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.

Mice given water bottle and empty bottle, alternating position.

In the afternoon (~4pm), mice will be given supplemental access to water for 30 min. Day 12: Conditioning Day (2023-3-16)

Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg

Groups:

• JCC01-04: Female, vehicle
• JCC05-08: Female, CNO
• JCC09-12: Male, vehicle
• JCC13-16: Male, CNO

After injection, ad lib water returned overnight.

Day 13 - 24: Preference Tests (2023-3-17 - 2023-3-24 )

CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.