PCR

Primers

To dilute primers to a stock of 100 µM, add 10 µl of water for each nmol of lyophilized primer. For example, if there are 38.2 nmol of primer a 100 µM primer stock is created by adding 382 µl of water.

Dilute 10 µM working stock aliquots from the 100µM stock 10-fold, e.g. 10 µl of 100µM and 90 µl of water.