FosCreER DREADD PCR

Extraction and Purification of DNA from Tail Biopsy

Tail Biopsy

Mice are first anesthetized in a bell jar with Isoflurane for ~20 sec, monitor animal closely and check with toe pinch before proceeding

Cycling Protocol

The below cycling protocol works for both DREADDs and the iCreER (TRAP2) allele.

1. 95.0° for 2 min
2. 95.0° for 20 sec
3. 65.0° for 15 sec, -0.5° per cycle
4. 72.0° for 10 sec
5. Goto 2, 10 times
6. 95.0° for 15 sec
7. 60.0° for 15 sec
8. 72.0° for 10 sec
9. Goto 6, 30 times
10. 72.0° for 5 min