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Line 39:
Line 39: − PMID 10493820 PMID 11577088+ − + − Sequence:+ − Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala- Asn-Met-Cys-Leu-Pro-OH + + + + − Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/]+ + + + +
→R18 peptide: added difopein and C54 info
==R18 peptide ==
==R18 peptide ==
PMID 11577088
R18 (aka difopein) is a synthetic unphosphorylated peptide (MW 2309.6) which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3.
R18 is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6) which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/].
Difopein is a construct with two R18 monomers in a single 62 amino acid peptide PMID 11577088
R18 Sequence:
Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala- Asn-Met-Cys-Leu-Pro-OH
PHCVPRDLSWLDLEANMCLP
PHCVPRDLSWLDLEANMCLP
Difopein sequence (with individual R18 monomers in parentheses):
SADGA (PHCVP RDLSW LDLEA NMCLP) GAAGL DSADG A(PHCV PRDLS WLDLE ANMCL P) GAAG LE
C54 (consisting of the carboyl-terminal 54-amino acid region of theHMG-box binding protein Ste11p ) is another 14-3-3 inhibitor that is structurally unrelated to R18/difopein. The C54 region permits sequestration of Ste11p in the cytoplasm to inhibit nuclear localization PMID 12697825.
==Thy-1 Promoter==
==Thy-1 Promoter==