Changes

==14-3-3==

==14-3-3==

https://ghr.nlm.nih.gov/gene/YWHAE

https://medlineplus.gov/genetics/gene/ywhae/

Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)

Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)

Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA

Mus musculus tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae), mRNA

NCBI Reference Sequence: NM_009536.4

NCBI Reference Sequence: NM_009536.4

==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-DIfopein) Transgenic Mice==

==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-DIfopein) Transgenic Mice==

PMID 11577088

PMID 11577088

R18  is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6)  which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/].

R18  is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6)  which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [https://www.enzolifesciences.com/BML-P214/r18-peptide/ Enzo #BML-P214-0001].

Difopein is a construct with two R18 monomers in a single 62 amino acid peptide   PMID 11577088. Commercial source of peptide: [Tocris #1245 http://www.tocris.com/dispprod.php?ItemId=110673]

Difopein is a construct with two R18 monomers in a single 62 amino acid peptide PMID 11577088. Commercial source of peptide: [https://www.tocris.com/products/difopein_2145 Tocris #1245]

==YFP==

==YFP==

YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).

YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.thermofisher.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).

== 14-3-3 FKO papers==

== 14-3-3 FKO papers==

Vogt, Peggy, [http://diginole.lib.fsu.edu/islandora/object/fsu:204445 "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.

Vogt, Peggy, [https://diginole.lib.fsu.edu/islandora/object/fsu:204445 "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.

C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400

C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400

==Antisera for IP Experiments==

==Antisera for IP Experiments==

anti-pan-14-3-3 (K-19) , rabbit polyclonal,  Santa Cruz #sc-629, 200 ug/1 ml,  $279 [https://www.scbt.com/scbt/product/pan-14-3-3-antibody-k-19 datasheet]

anti-pan-14-3-3 (K-19) , rabbit polyclonal,  Santa Cruz #sc-629, 200 ug/1 ml,  $279 [https://www.scbt.com/p/pan-14-3-3-antibody-k-19 datasheet]

anti-HDAC4 (H-92), rabbit polyclonal,  Santa Cruz #sc-11418, 200 ug/1 ml, $279 [https://www.scbt.com/scbt/product/hdac4-antibody-h-92 datasheet]

anti-HDAC4 (H-92), rabbit polyclonal,  Santa Cruz #sc-11418, 200 ug/1 ml, $279 [https://www.scbt.com/p/hdac4-antibody-h-92 datasheet]

anti-Torc1 (C71D11), rabbit mAb, Cell Signaling  #2587 $225 [https://www.cellsignal.com/products/primary-antibodies/torc1-crtc1-c71d11-rabbit-mab/2587 datasheet]

anti-Torc1 (C71D11), rabbit mAb, Cell Signaling  #2587 $225 [https://www.cellsignal.com/products/primary-antibodies/torc1-crtc1-c71d11-rabbit-mab/2587?Ntk=Products&Ntt=2587 datasheet]