DMC Sodium Butyrate and Flavor Conditioning
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances preference learning
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
Data
People
Durba Mukherjee, Alex Haimovich
Agenda
n=16 adult male sprague-dawley rats (Charles River CD), individually housed in BRF 169
Days 0-8: Food and water restricted. (October 8 - October 16, 2019)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
Water removed on Tuesday Oct 8, 2019. 30 min water at 1pm daily, pellet ration at 1:30pm
Day 9: Conditioning Day 1 (October 17, 2019)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
30 min: remove CS+, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, give daily pellet ration
- Rats 1-8: grape CS+, Rats 9-16: Cherry CS+.
- Odd rats: NaCL injection. Even rats: NaB injection
Day 10: Conditioning Day 2 (October 18, 2019)
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape)
30 min: remove CS-, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, return food and water ad lib
- Rats 1-8: Cherry CS-, Rats 9-16: Grape CS-.
- Odd rats: NaCL injection. Even rats: NaB injection
Days 11-17: 2 bottle preference tests (October 19-25, 2019)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.