Difference between revisions of "DME Sodium Butyrate and Flavor Conditioning 2"
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Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | ||
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| + | * 0.3 M Na Butyrate = 3.3g/100ml water | ||
| + | * 0.3 M NaCl = 18g/liter water (osmotic control) | ||
==Data== | ==Data== | ||
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==Agenda== | ==Agenda== | ||
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<br> | <br> | ||
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n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169. | n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169. | ||
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| + | <br> | ||
'''Days 0: Food and water removed.''' (July 10, 2020) | '''Days 0: Food and water removed.''' (July 10, 2020) | ||
| − | '''Days 1: Food and water restricted.''' (July 11 - July 17, 2020) | + | <br> |
| + | '''Days 1-7: Food and water restricted.''' (July 11 - July 17, 2020) | ||
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight. | Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight. | ||
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<br> | <br> | ||
| − | '''Day | + | '''Day 8: Conditioning Day 1''' (July 18, 2020) |
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am | 0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am | ||
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<br> | <br> | ||
| − | '''Day | + | '''Day 9: Conditioning Day 2''' (July 19, 2020) |
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am | 0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am | ||
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<br> | <br> | ||
| − | '''Days | + | '''Days 10-18: 2 bottle preference tests''' (July 20 - 28, 2020) |
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day. | Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day. | ||
<br> | <br> | ||
| + | |||
| + | |||
| + | '''Notes:''' | ||
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| + | * Rat DME 16 did not drink from CS+/glucose on conditioning day 1 | ||
| + | * CS- bottles not weighed in conditioning day 2, so estimated volumetrically from fill lines | ||
<br> | <br> | ||
| − | + | <br> | |
| + | <br> | ||
[[Category:Flavor Preference]] | [[Category:Flavor Preference]] | ||
Revision as of 11:34, 19 July 2020
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances preference learning
Replication of [DMC Sodium Butyrate and Flavor Conditioning] with following modifications:
1. To prevent formation of side preferencs, rats were trained with 2 choice water and 0.05% saccharin prior to conditioning
2. To prevent ceiling effect of preferences being too strong, CS+ used only 4% glucose
3. n=9 male, n=9 female, counterbalanced
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
Agenda
Rats arrive. (July 7, 2020)
n=18 adult male and female sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Days 0: Food and water removed. (July 10, 2020)
Days 1-7: Food and water restricted. (July 11 - July 17, 2020)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
30 min water at 1100am daily, pellet ration at 1130am
Day 8: Conditioning Day 1 (July 18, 2020)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 4% glucose (counterbalanced cherry, grape) at 1135am - 1155am
30 min: remove CS+, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl, 1200am-1213am
Give daily pellet ration at 1215am
- Rats 1-9 : female, Rats 10-18: male
- Rats 1,2,5,6,9,10,13,14,17,18: Cherry CS+
- Rats 3,4,7,8,11,12,15,16: Grape CS+
- Odd rats: NaB injection; Even rats: NaCL injection
Day 9: Conditioning Day 2 (July 19, 2020)
0 min: 30 min access to CS- 005% Kool-Aid + 0.05% saccharin (counterbalanced cherry, grape) at 1106-1136am
CS- bottles not weighed, so estimated volumetrically from fill lines.
30 min: remove CS-, inject with 12ml/kg of 0.3M sodium butyrate or 12ml/kg of 0.3M NaCl,
return food and water ad lib
- Rats 1-9 : female, Rats 10-18: male
- Rats 1,2,5,6,9,10,13,14,17,18: Grape CS-
- Rats 3,4,7,8,11,12,15,16: Cherry CS-.
- Odd rats: NaB injection; Even rats: NaCL injection.
Days 10-18: 2 bottle preference tests (July 20 - 28, 2020)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.
Notes:
- Rat DME 16 did not drink from CS+/glucose on conditioning day 1
- CS- bottles not weighed in conditioning day 2, so estimated volumetrically from fill lines