Difference between revisions of "DMF Sodium Butyrate and Saccharin-LiCl CTA"
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Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning . | Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning . | ||
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| + | (see replication to increase N at [[DMG Sodium Butyrate and Saccharin-LiCl CTA]]) | ||
| + | <br> | ||
A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]. | A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]. | ||
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ad lib water returned after final injection overnight. | ad lib water returned after final injection overnight. | ||
| − | * Rats 1-8 : male, Rats | + | * Rats 1-8 : male, Rats 9-16: female |
* All rats: saccharin | * All rats: saccharin | ||
* All rats: LiCl (0.15M, 3ml/kg) | * All rats: LiCl (0.15M, 3ml/kg) | ||
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| − | '''Days 13 - | + | '''Days 13 - 22: Preference Tests''' (October 22 - November 1, 2020) |
| − | Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both | + | Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days. |
[[Category:Flavor Preference]] | [[Category:Flavor Preference]] | ||
[[Category:Taste Aversion]] | [[Category:Taste Aversion]] | ||
Revision as of 11:06, 24 November 2020
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
(see replication to increase N at DMG Sodium Butyrate and Saccharin-LiCl CTA)
A more strict replication of Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962.
1. Ordered brand new Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).
2. n=8 male, n=8 female, counterbalanced
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
CTA Conditioning
Agenda
Rats arrive (October 6, 2020) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed (October 9, 2020)
Days 1 - 11: Water Restriction (October 10 - October 20, 2020)
Weigh rats daily. water for 10 min, ad lib food.
10 min water at 1430 daily.
Day 12: Conditioning Day (October 21, 2020)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M LiCl, 3 ml/kg ip. 40 min: odd rats injected with 0.3 M NaCl 12 ml/kg ip; even rats injected with 0.3 M Na Butyrate 12 ml/kg, ip
ad lib water returned after final injection overnight.
- Rats 1-8 : male, Rats 9-16: female
- All rats: saccharin
- All rats: LiCl (0.15M, 3ml/kg)
- Odd rats: NaCL injection; Even rats: NaB injection;
Days 13 - 22: Preference Tests (October 22 - November 1, 2020)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.