DMF Sodium Butyrate and Saccharin-LiCl CTA
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
1. Ordered brand new Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).
2. n=8 male, n=8 female, counterbalanced
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Rats arrive (October 6, 2020) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed (October 9, 2020)
Days 1 - 11: Water Restriction (October 10 - October 20, 2020)
Weigh rats daily. water for 10 min, ad lib food.
10 min water at 1430 daily.
Day 12: Conditioning Day (October 21, 2020)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M LiCl, 3 ml/kg ip. 40 min: odd rats injected with 0.3 M NaCl 12 ml/kg ip; even rats injected with 0.3 M Na Butyrate 12 ml/kg, ip
ad lib water returned after final injection overnight.
- Rats 1-8 : male, Rats 10-18: female
- All rats: saccharin
- All rats: LiCl (0.15M, 3ml/kg)
- Odd rats: NaCL injection; Even rats: NaB injection;
Days 13 - ??: Preference Tests (October 22 - ?? October , 2020)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 75 mM NaCl and water bottles, alternating position for 6 days.