Difference between revisions of "DMH Sodium Butyrate and Saccharin CTA Controls"

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Replication (to increase N) of [[DMF Sodium Butyrate and Saccharin-LiCl CTA]] and [[DMG Sodium Butyrate and Saccharin-LiCl CTA]] with NaCl-conditioned control groups.
  
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Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
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A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962].
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1. Sodium Butyrate ([https://www.sigmaaldrich.com/catalog/search?term=303410 Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ).
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2. n=8 male, n=8 female, counterbalanced
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Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
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* 0.3 M Na Butyrate = 3.3g/100ml water
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* 0.3 M NaCl = 18g/liter water (osmotic control)
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<br>
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==Data==
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'''[https://houptlab.org/bartab/expt/?id=DMH Raw Data Link]'''
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==People==
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Durba Mukherjee
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==CTA Conditioning==
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===Agenda===
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<br>
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'''Rats arrive''' (January 12, 2021)
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n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
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<br>
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'''Day 0: Water removed''' (January 19, 2021)
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<br>
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'''Days 1 - 7: Water Restriction''' (January 20 - January 26, 2021)
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Weigh rats daily.  water for 10 min, ad lib food.
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10 min water at 1430 daily.
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<br>
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'''Day 8: Conditioning Day'''  (January 27 2021)
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0 min: 30 min access to 0.125% saccharin  (5 g sodium saccharin/4L water)
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30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
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40 min:  half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip
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ad lib water returned after final injection overnight.
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* Rats 1-8 : female, Rats 9-16: male
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* All rats: saccharin
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* All rats: NaCl (0.15M, 3ml/kg)
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* rats 1-4, 9-12:  NaCL injection; rats 5-8,13-16: NaB injection;
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'''Days 9 - ??: Preference Tests''' (January 28 - ?? February  , 2021)
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Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
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[[Category:Flavor Preference]]
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[[Category:Taste Aversion]]

Revision as of 14:12, 27 January 2021

Replication (to increase N) of DMF Sodium Butyrate and Saccharin-LiCl CTA and DMG Sodium Butyrate and Saccharin-LiCl CTA with NaCl-conditioned control groups.

Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .

A more strict replication of Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962.

1. Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).

2. n=8 male, n=8 female, counterbalanced


Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962

  • 0.3 M Na Butyrate = 3.3g/100ml water
  • 0.3 M NaCl = 18g/liter water (osmotic control)


Data

Raw Data Link

People

Durba Mukherjee


CTA Conditioning

Agenda


Rats arrive (January 12, 2021) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.


Day 0: Water removed (January 19, 2021)


Days 1 - 7: Water Restriction (January 20 - January 26, 2021)

Weigh rats daily. water for 10 min, ad lib food.

10 min water at 1430 daily.


Day 8: Conditioning Day (January 27 2021)

0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip

ad lib water returned after final injection overnight.

  • Rats 1-8 : female, Rats 9-16: male
  • All rats: saccharin
  • All rats: NaCl (0.15M, 3ml/kg)
  • rats 1-4, 9-12: NaCL injection; rats 5-8,13-16: NaB injection;


Days 9 - ??: Preference Tests (January 28 - ?? February , 2021)

Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.

Retrieved from "https://wiki.houptlab.org/index.php?title=DMH_Sodium_Butyrate_and_Saccharin_CTA_Controls&oldid=2762"