Difference between revisions of "DMH Sodium Butyrate and Saccharin CTA Controls"

From MagnetoWiki
Jump to navigation Jump to search
(created page)
 
(Updated Sodium Butyrate link.)
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 +
Replication (to increase N) of [[DMF Sodium Butyrate and Saccharin-LiCl CTA]] and [[DMG Sodium Butyrate and Saccharin-LiCl CTA]] with NaCl-conditioned control groups.
  
 +
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
 +
 +
A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962].
 +
 +
1. Sodium Butyrate ([https://www.sigmaaldrich.com/US/en/product/aldrich/303410 Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ).
 +
 +
2. n=8 male, n=8 female, counterbalanced
 +
 +
 +
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
 +
 +
* 0.3 M Na Butyrate = 3.3g/100ml water
 +
* 0.3 M NaCl = 18g/liter water (osmotic control)
 +
 +
<br>
 +
 +
==Data==
 +
 +
'''[https://houptlab.org/bartab/expt/?id=DMH Raw Data Link]'''
 +
 +
==People==
 +
 +
Durba Mukherjee
 +
 +
 +
==CTA Conditioning==
 +
 +
===Agenda===
 +
 +
<br>
 +
 +
'''Rats arrive''' (January 12, 2021)
 +
n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
 +
 +
<br>
 +
 +
'''Day 0: Water removed''' (January 19, 2021)
 +
 +
<br>
 +
 +
'''Days 1 - 7: Water Restriction''' (January 20 - January 26, 2021)
 +
 +
Weigh rats daily.  water for 10 min, ad lib food.
 +
 +
10 min water at 1430 daily.
 +
 +
<br>
 +
 +
'''Day 8: Conditioning Day'''  (January 27 2021)
 +
 +
0 min: 30 min access to 0.125% saccharin  (5 g sodium saccharin/4L water)
 +
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
 +
40 min:  half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip
 +
 +
ad lib water returned after final injection overnight.
 +
 +
* Rats 1-8 : female, Rats 9-16: male
 +
* All rats: saccharin
 +
* All rats: NaCl (0.15M, 3ml/kg)
 +
* rats 1-4, 9-12:  NaCL injection; rats 5-8,13-16: NaB injection;
 +
 +
 +
'''Days 9 - ??: Preference Tests''' (January 28 - ?? February  , 2021)
 +
 +
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
 +
 +
 +
[[Category:Flavor Preference]]
 +
[[Category:Taste Aversion]]

Revision as of 08:49, 22 June 2021

Replication (to increase N) of DMF Sodium Butyrate and Saccharin-LiCl CTA and DMG Sodium Butyrate and Saccharin-LiCl CTA with NaCl-conditioned control groups.

Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .

A more strict replication of Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962.

1. Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).

2. n=8 male, n=8 female, counterbalanced


Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962

  • 0.3 M Na Butyrate = 3.3g/100ml water
  • 0.3 M NaCl = 18g/liter water (osmotic control)


Data

Raw Data Link

People

Durba Mukherjee


CTA Conditioning

Agenda


Rats arrive (January 12, 2021) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.


Day 0: Water removed (January 19, 2021)


Days 1 - 7: Water Restriction (January 20 - January 26, 2021)

Weigh rats daily. water for 10 min, ad lib food.

10 min water at 1430 daily.


Day 8: Conditioning Day (January 27 2021)

0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip

ad lib water returned after final injection overnight.

  • Rats 1-8 : female, Rats 9-16: male
  • All rats: saccharin
  • All rats: NaCl (0.15M, 3ml/kg)
  • rats 1-4, 9-12: NaCL injection; rats 5-8,13-16: NaB injection;


Days 9 - ??: Preference Tests (January 28 - ?? February , 2021)

Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.

Retrieved from "https://wiki.houptlab.org/index.php?title=DMH_Sodium_Butyrate_and_Saccharin_CTA_Controls&oldid=2796"