DMW Ovariectomy and Butyrate-Enhanced CTA 4
Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of DMP Ovariectomy and Butyrate-Enhanced CTA.
| Ovariectomy and Butyrate-Enhanced CTA 4 | |
|---|---|
| HDAC Sex Difference | |
| Expt Code | DMW |
| Start Date | 2023-6-18 |
| Investigators | Houpt, Bass, Cote, D. Jorge |
| Funding | Chair's Fund |
| Protocol | 202200000006 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMW |
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
Agenda
Rats arrive Female Sprague Dawley rats n = XXX, d.o.b. XXXX, arrived: XXXX
Surgery
all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
- DMW 1-XXX : Surgery on 2023-
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'Day 0: Water removed XXXX
Days 1-8: Water Restriction XXXX thru XXXX
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
Day 9: Conditioning Day XXXX
- 0-30 min: saccharin 0.125%
- at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
- at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
Groups:
- OVX + LiCl + NaCl: DMW XXX
- OVX + LiCl + Butyrate: DMW XXX
- Sham + LiCl + NaCl: DMW XXX
- Sham + LiCl + Butyrate: DMW XXX
Days 10 - 19: Preference Tests: XXXX to XXXX
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.