MBC Selenate and CFP 3

This is the third replicate of selenate pre-Rx before glucose-induced flavor preference conditioning

Selenate Dosage

To test selenate attenuation of CFNP, rats will be injected with 1 ml/kg of 0.5 mg/ml Na2SeO4 in 0.15 M NaCl, (so 0.5 mg/kg selenate), 2 hours before conditioning.

• Sodium Selenate Na2SeO4 from 2021-2-16 from [Millipore-Sigma S8295-25G

• MW 188.95, CAS Number 13410-01-0, PubChem Substance ID 24899787

• 0.5 mg/ml Na2Se04 is 2.65 mM, or 7.95 mOsm (assuming complete dissociation into 2 Na+ and 1 SeO4- ions)

• So OK to mix 0.5 mg Na2SeO4 in 1 ml of 0.15 M NaCl, resulting in a 308 mOsm solution that is still near isotonic (300mOsM).

Data

Raw Data Link

People

Marena Bass

Agenda

Day 0 (04/29/2021): At 5pm, adult male Sprague-Dawley rats (N=16) were weighed to determine their initial body weight, and their food and water was removed to begin the food and water restriction schedule.

Days 1-8 (04/30/2021-05/07/2021): Food/water restriction and training

• The rats were trained on a restricted food and water schedule for 8 days before conditioning.
• Each day at 1pm, the animals were weighed and given 23-25 grams of chow to maintain 90% body weight.
• After being weighed, animals were given access to two bottles, 0.05% saccharin and water. Initially, they were given 1hr to drink the solutions, and this was gradually reduced to 30 mins per day. Bottle positions were alternated each day.

Day 9 (05/08/2021): CS+ Conditioning

• 11 am: Animals injected (IP) with 1 mL per kg body weight of 0.15M NaCl or 0.5mg/mL sodium selenate
• 1pm: Animals given 30min access to CS+ containing 4% glucose, 0.05% saccharin and 0.05% grape or cherry flavored Kool-Aid (flavors counterbalanced)
• 23-25g food ration given after CS+ removed

Day 10 (05/09/2021): CS- Conditioning

• 11 am: Animals injected (IP) with 1 mL per kg body weight of 0.15M NaCl or 0.5mg/mL sodium selenate
• 1pm: Animals given 30min access to CS- containing, 0.05% saccharin and 0.05% Kool-Aid (whichever flavor the animal did not receive yesterday)
• Ad. lib. water returned to cages and 23-25g food ration given after CS- removed

Days 11-20 (05/10/2021-05/20/2021): 10 days of 2-bottle preference testing

• Each day, animals were given ad. lib. food and 24 hr access to both the CS- and the CS+ (without glucose) solutions. Bottle positions were alternated each day (i.e., CS+ on right for day 11 and CS+ on left for day 12, etc.)