MBC Selenate and CFP 3
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
This is the third replicate of selenate pre-Rx before glucose-induced flavor preference conditioning
Selenate Dosage
To test selenate attenuation of CFNP, rats will be injected with 1 ml/kg of 0.5 mg/ml Na2SeO4 in 0.15 M NaCl, (so 0.5 mg/kg selenate), 2 hours before conditioning.
- Sodium Selenate Na2SeO4 from 2021-2-16 from Millipore-Sigma S8295-25G
- MW 188.95, CAS Number 13410-01-0, PubChem Substance ID 24899787
- 0.5 mg/ml Na2Se04 is 2.65 mM, or 7.95 mOsm (assuming complete dissociation into 2 Na+ and 1 SeO4- ions)
- So OK to mix 0.5 mg Na2SeO4 in 1 ml of 0.15 M NaCl, resulting in a 308 mOsm solution that is still near isotonic (300mOsM).
Data
People
Marena Bass
Agenda
Day 0 (04/29/2021): At 5pm, adult male Sprague-Dawley rats (N=16) were weighed to determine their initial body weight, and their food and water was removed to begin the food and water restriction schedule.
Days 1-8 (04/30/2021-05/07/2021): Food/water restriction and training
- The rats were trained on a restricted food and water schedule for 8 days before conditioning.
- Each day at 1pm, the animals were weighed and given 23-25 grams of chow to maintain 90% body weight.
- After being weighed, animals were given access to two bottles, 0.05% saccharin and water. Initially, they were given 1hr to drink the solutions, and this was gradually reduced to 30 mins per day. Bottle positions were alternated each day.
Day 9 (05/08/2021): CS+ Conditioning
- 11 am: Animals injected (IP) with 1 mL per kg body weight of 0.15M NaCl or 0.5mg/mL sodium selenate
- 1pm: Animals given 30min access to CS+ containing 4% glucose, 0.05% saccharin and 0.05% grape or cherry flavored Kool-Aid (flavors counterbalanced)
- 23-25g food ration given after CS+ removed
Day 10 (05/09/2021): CS- Conditioning
- 11 am: Animals injected (IP) with 1 mL per kg body weight of 0.15M NaCl or 0.5mg/mL sodium selenate
- 1pm: Animals given 30min access to CS- containing, 0.05% saccharin and 0.05% Kool-Aid (whichever flavor the animal did not receive yesterday)
- Ad. lib. water returned to cages and 23-25g food ration given after CS- removed
Days 11-20 (05/10/2021-05/20/2021): 10 days of 2-bottle preference testing
- Each day, animals were given ad. lib. food and 24 hr access to both the CS- and the CS+ (without glucose) solutions. Bottle positions were alternated each day (i.e., CS+ on right for day 11 and CS+ on left for day 12, etc.)