Methylation-Sensitive Enzymes
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Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
| Enzyme NEB link Site Cut Position Reaction Conditions |
|---|
| HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3' 3’...GGC/C...5’ 1X NEBuffer 1, Incubate at 37°C. |
| MspI https://www.neb.com/products/r0106-mspi CCgg 5’...C/CGG...3' 3’...GGC/C...5’ 1X NEBuffer 4, Incubate at 37°C. |
| AciI https://www.neb.com/products/r0551-aciip CCgC 5’...C/CGC...3' 3’...GGC/G...5’ 1X NEBuffer 3, Incubate at 37°C. |
| HhaI https://www.neb.com/products/r0139-hhai gCgC 5’...GCG/C...3' 3’...C/GCG...5’ 1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C. |