Difference between revisions of "NMDA Receptors"
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| + | =Primers for Davenport's Real-Time PCR experiment= | ||
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| + | Primers were ordered 2009 March from Sigma Aldrich | ||
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| + | <tab class="wikitable" border = "1" cellspacing="3" cellpadding = "3" head = top> | ||
| + | Probe Abbr Sequence Tm Size Genbank Reference | ||
| + | NR1 RTNR1FOR GTTCTTCCGCTCAGGCTTTG 66.0 66 RNU11418, 2396-2461 Yamamoto et al. 2008 PMID 17957211 | ||
| + | RTNR1REV AGGGAAACGTTCTGCTTCCA 65.8 | ||
| + | Serine Racemase RTSERRACFOR ATTGCAAGAAACTGGCCATC 63.9 295 NM198757, 664-958 Yoshikawa et al., 2006 PMID 16973158 | ||
| + | RTSERRACREV TCAGCAGCGTACACCTTCAC 64.1 | ||
| + | GADPH RTGADPHFOR TGCACCACCAACTGCTTAG 62.5 201 DQ403053.1, 397-597 Uno et al., 2002 PMID 12068078 | ||
| + | RTGADPHREV GGATGCAGGGATGATGTTC 63.0 | ||
| + | Note: GADPHFOR and REV maybe mixed up . . . . | ||
| + | </tab> | ||
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=Primers for Real-Time PCR= | =Primers for Real-Time PCR= | ||
Revision as of 14:09, 30 March 2009
Primers for Davenport's Real-Time PCR experiment
Primers were ordered 2009 March from Sigma Aldrich
| Probe Abbr Sequence Tm Size Genbank Reference |
|---|
| NR1 RTNR1FOR GTTCTTCCGCTCAGGCTTTG 66.0 66 RNU11418, 2396-2461 Yamamoto et al. 2008 PMID 17957211 |
| RTNR1REV AGGGAAACGTTCTGCTTCCA 65.8 |
| Serine Racemase RTSERRACFOR ATTGCAAGAAACTGGCCATC 63.9 295 NM198757, 664-958 Yoshikawa et al., 2006 PMID 16973158 |
| RTSERRACREV TCAGCAGCGTACACCTTCAC 64.1 |
| GADPH RTGADPHFOR TGCACCACCAACTGCTTAG 62.5 201 DQ403053.1, 397-597 Uno et al., 2002 PMID 12068078 |
| RTGADPHREV GGATGCAGGGATGATGTTC 63.0 |
| Note: GADPHFOR and REV maybe mixed up . . . . |
Primers for Real-Time PCR
Taken from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems
NR1
Forward primer 5'-GTTCTTCCGCTCAGGCTTTG-3' Reverse primer 5'-AGGGAAACGTTCTGCTTCCA-3' TaqMan probe 5'-FAM-CGGCATGCGCAAGGACAGCC-TAMRA-3'
NR2A
Forward primer 5'-AGCCCCCTTCGTCATCGTA-3' Reverse primer 5'-GACAGGGCACCGTGTTCCT-3' TaqMan probe 5'-FAM-AGGACATAGACCCCCTGACTGAGACCTGTG-TAMRA-3'
NR2B
Forward primer 5'-CCCCCAAGTTCTGGTTGGT-3' Reverse primer 5'-TTTTGGGAACGAGCTTTGCT-3' TaqMan probe 5'-FAM-TTGGCCGTCTTGGCCGTATCAGG-TAMRA-3'
NR2C
Forward primer 5'-GGGTGATGATGTTCGTGATGTG-3' Reverse primer 5'-GTGAGGTTCTGGTTGTAGCTGACA-3' TaqMan probe 5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3'