Revision as of 13:39, 12 December 2006

Serine Racemase Probe for in situ hybridization

Primer sequences taken from Yoshikawa et al., PMID 16973158

Rat serine racemase. Genbank Ascension # NM_198757

PCR product Length: 295 bases (+22 bases for T7 promoter]

Forward Primer 664 to 683

ATT GCA AGA AAC TGG CCA TC

Reverse Primer [with T7 promoter] 958 to 939

[CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC

Amplification conditions (from Yoshikawa et al, PMID 15337321):

10-min predenaturation at 95 °C

15-s denaturation at 95 °C.

20-s at 58 °C for serine racemase

followed by a 20-s extension at 72 °C.

@@ Line 6: / Line 6: @@
 PCR product Length: 295 bases (+22 bases for T7 promoter]
 Forward Primer 664 to 683
-ATT GCA AGA AAC TGG CCA TC
+:ATT GCA AGA AAC TGG CCA TC
 Reverse Primer [with T7 promoter]  958 to 939
-[CTT AAT ACG ACT CAC TAT  AGG G] TC AGC AGC GTA CAC CTT CAC
+:[CTT AAT ACG ACT CAC TAT  AGG G] TC AGC AGC GTA CAC CTT CAC
-*PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
+Amplification conditions (from Yoshikawa et al, PMID 15337321):
-	**10-min predenaturation at 95 °C
+:10-min predenaturation at 95 °C
-	**15-s denaturation at 95  °C.
+:15-s denaturation at 95  °C.
-	**20-s at 58 °C for serine racemase
+:20-s at 58 °C for serine racemase
-	**followed by a 20-s extension at 72  °C.
+:followed by a 20-s extension at 72  °C.