Difference between revisions of "Serine Racemase"
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| − | =Serine Racemase Probe for in situ hybridization= | + | ==Serine Racemase Probe for in situ hybridization== |
Primer sequences taken from Yoshikawa et al., PMID 16973158 | Primer sequences taken from Yoshikawa et al., PMID 16973158 | ||
Revision as of 13:40, 12 December 2006
Serine Racemase Probe for in situ hybridization
Primer sequences taken from Yoshikawa et al., PMID 16973158
Rat serine racemase. Genbank Ascension # NM_198757
PCR product Length: 295 bases (+22 bases for T7 promoter]
Forward Primer 664 to 683
- ATT GCA AGA AAC TGG CCA TC
Reverse Primer [with T7 promoter] 958 to 939
- [CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC
Amplification conditions (from Yoshikawa et al, PMID 15337321):
- 10-min predenaturation at 95 °C
- 15-s denaturation at 95 °C.
- 20-s at 58 °C for serine racemase
- followed by a 20-s extension at 72 °C.