Difference between revisions of "Serine Racemase"
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[CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC | [CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC | ||
| − | PCR amplification conditions (from Yoshikawa et al, PMID 15337321): | + | *PCR amplification conditions (from Yoshikawa et al, PMID 15337321): |
| − | *10-min predenaturation at 95 °C | + | **10-min predenaturation at 95 °C |
| − | *15-s denaturation at 95 °C. | + | **15-s denaturation at 95 °C. |
| − | *20-s at 58 °C for serine racemase | + | **20-s at 58 °C for serine racemase |
| − | *followed by a 20-s extension at 72 °C. | + | **followed by a 20-s extension at 72 °C. |
Revision as of 13:36, 12 December 2006
Serine Racemase Probe for in situ hybridization
Primer sequences taken from Yoshikawa et al., PMID 16973158
Rat serine racemase. Genbank Ascension # NM_198757
PCR product Length: 295 bases (+22 bases for T7 promoter] Forward Primer 664 to 683 ATT GCA AGA AAC TGG CCA TC
Reverse Primer [with T7 promoter] 958 to 939 [CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC
- PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
**10-min predenaturation at 95 °C **15-s denaturation at 95 °C. **20-s at 58 °C for serine racemase **followed by a 20-s extension at 72 °C.