Serine Racemase Probe for in situ hybridization

Primer sequences taken from Yoshikawa et al., PMID 16973158

Rat serine racemase. Genbank Ascension # NM_198757

PCR product Length: 295 bases (+22 bases for T7 promoter]

Forward Primer 664 to 683

ATT GCA AGA AAC TGG CCA TC

Reverse Primer [with T7 promoter] 958 to 939

[CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC

Amplification conditions (from Yoshikawa et al, PMID 15337321):

10-min predenaturation at 95 °C

15-s denaturation at 95 °C.

20-s at 58 °C for serine racemase

followed by a 20-s extension at 72 °C.

Serine Racemase