Serine Racemase Probe for in situ hybridization
Primer sequences taken from Yoshikawa et al., PMID 16973158
Rat serine racemase. Genbank Ascension # NM_198757
PCR product Length: 295 bases (+22 bases for T7 promoter] Forward Primer 664 to 683 ATT GCA AGA AAC TGG CCA TC
Reverse Primer [with T7 promoter] 958 to 939 [CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC
- PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
**10-min predenaturation at 95 °C **15-s denaturation at 95 °C. **20-s at 58 °C for serine racemase **followed by a 20-s extension at 72 °C.