Changes

==14-3-3==

==14-3-3==

http://ghr.nlm.nih.gov/gene/YWHAE

https://medlineplus.gov/genetics/gene/ywhae/

Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)

Ywhae gene = 14-3-3 epsilon = tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (Ywhae)

NCBI Reference Sequence: NM_009536.4

NCBI Reference Sequence: NM_009536.4

 

==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-DIfopein) Transgenic Mice==

 

==Dr. Yi Zhou's 14-3-3 FKO (Thy1-YFP-R18) Transgenic Mice==

See SFN abstracts (2010) 53.1/M17 and (2011) 790.09/FF16

See SFN abstracts (2010) 53.1/M17 and (2011) 790.09/FF16

From Sun et al PMID 23861400 :

From Sun et al PMID 23861400 :

Glutamatergic kainate receptors (KAR) KAR-EPSCs at mossy fiber-CA3 synapses decay significantly faster in the 14-3-3 functional knock-out mice (but no difference in NMDA-R or AMPA-R EPSC in same synapses)  

Glutamatergic kainate receptors (KAR) KAR-EPSCs at mossy fiber-CA3 synapses decay significantly faster in the 14-3-3 functional knock-out mice (but no difference in NMDA-R or AMPA-R EPSC in same synapses)

PMID 10493820 PMID 11577088

R18  is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6)  which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [https://www.enzolifesciences.com/BML-P214/r18-peptide/ Enzo #BML-P214-0001].

R18 (aka difopein) is a synthetic unphosphorylated peptide (MW 2309.6)  which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3.  

Difopein is a construct with two R18 monomers in a single 62 amino acid peptide PMID 11577088. Commercial source of peptide: [https://www.tocris.com/products/difopein_2145 Tocris #1245]

  PHCVPRDLSWLDLEANMCLP

  PHCVPRDLSWLDLEANMCLP

Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/]

 

 

C54 (consisting of the carboyl-terminal 54-amino acid region of theHMG-box binding protein Ste11p ) is another 14-3-3 inhibitor that is structurally unrelated to R18/difopein. The C54 region permits sequestration of Ste11p in the cytoplasm to inhibit nuclear localization PMID 12697825.

==Thy-1 Promoter==

==Thy-1 Promoter==

==YFP==

==YFP==

YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. R18 is 21 amino acids long (63 bp), so total YFP-R18 fusion protein should be ~ 260 amino acids (780 bp).

YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.thermofisher.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).

== 14-3-3 FKO papers==

== 14-3-3 FKO papers==

Vogt, Peggy, [http://diginole.lib.fsu.edu/uhm/42/ "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.

Vogt, Peggy, [https://diginole.lib.fsu.edu/islandora/object/fsu:204445 "Exploring the Function of 14-3-3 Proteins in Neurodegenerative Disease"] (2011). Honors Theses. Paper 42.

C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400

C. Sun, H. Qiao, Q. Zhou, Y. Wang, Y. Wu, Y. Zhou, et al., Modulation of GluK2a Subunit-containing Kainate Receptors by 14-3-3 Proteins, J Biol Chem. 288 (2013) 24676–24690. PMID 23861400

Qiao H, Foote M, Graham K, Wu Y, Zhou Y. 14-3-3 proteins are required for hippocampal long-term potentiation and associative learning and memory. J Neurosci. 34(2014):4801-8. PMID 24695700

Qiao H, Foote M, Graham K, Wu Y, Zhou Y. 14-3-3 proteins are required for hippocampal long-term potentiation and associative learning and memory. J Neurosci. 34(2014):4801-8. PMID 24695700