TESS

TESS is the Transcription Element Search System curated by the University of Pennsylvania.

TESS is a web tool for predicting transcription factor binding sites in DNA sequences. It can identify binding sites using site or consensus strings and positional weight matrices from the TRANSFAC, JASPAR, IMD, and our CBIL-GibbsMat database. You can use TESS to search a few of your own sequences or for user-defined CRMs genome-wide near genes throughout genomes of interest.

Citation

To cite the use of TESS, cite the following technical report:

Schug, J. and C.G. Overton. TESS: Transcription element search software on the WWW. Technical Report CBIL-TR-1997-1001-v0.0, Computational Biology and Informatics Laboratory, School of Medicine, University of Pennsylvania, 1997, URL: http://www.cbil.upenn.edu/tess

or cite the following book chapter:

Schug, Jonathan,Using TESS to Predict Transcription Factor Binding Sites in DNA Sequence in Current Protocols in Bioinformatics, ed. A.D. Baxevanis. J Wiley & Sons, 2003.

Interpreting Results

Here is the description for the scores returned by TESS. Essentially, sort the Tabular Results using the "La" column to get the best fitting results (i.e. most likely to be real matches of the cis-element with your sequence. ) Those items with an "La/Len" score of 2.0 are perfect fits.

Also, it is presumably a good idea to get p-values less than 0.05 for Lpv, Spv, and Pv, although I'm not sure what that means.

La Log-likelihood score, higher is better.

La/ La / Len, higher is better, maximum is 2.0.

Lq La / L_M, where L_M is the maximum La possible for the site model, higher is better, best is 1.0

Ld L_M - La, 0 is best, higher is worse.

L Pv Approximate p-value for La score

Sc Core similarity as reported at TRANSFAC site

Sm Matrix similarity as reported at TRANSFAC site

S Pv Approximate p-value for Sm score

P-v Poisson-model p-value

Model Which site strings or weight matrix was used to pick this site

Factor Which factor(s) does the model represent